A disulfide conjugate between anti-tetanus antibodies and HIV (37-72)Tat neutralizes tetanus toxin inside chromaffin cells

Comparative in vitro and in vivo assessment of toxin neutralization by anti-tetanus toxin monoclonal antibodies.

Tetanus is caused by the tetanus neurotoxin (TeNT), a 150 kDa single polypeptide molecule which is cleaved into an active two-chain molecule composed of a 50 kDa N-terminal light (L) and a 100 kDa C-terminal heavy (H) chains. Recently, extensive effort has focused on characterization of TeNT binding receptors and toxin neutralization by monoclonal antibodies (mAbs). Toxin binding inhibition and...

Effects of tetanus toxin on catecholamine release from intact and digitonin-permeabilized chromaffin cells.

Tetanus exotoxin inhibited Ca2+-dependent catecholamine secretion in a dose-dependent manner in digitonin-permeabilized chromaffin cells. The inhibition was specific for tetanus exotoxin and the B fragment of tetanus toxin; the C fragment had no effect. Inhibition required the introduction of toxin into the cell, and was not seen when intact cells were preincubated with the toxin or toxin fragm...

Structure of Tetanus Toxin DEMONSTRATION AND SEPARATION OF A SPECIFIC ENZYME CONVERTING INTRACELLULAR TETANUS TOXIN

Protease activity has been demonstrated in culture supernatants of Clostridium tetani at various stages of fermentation. Gel chromatography of the concentrated filtrates revealed the presence of three enzymatically active fractions eluting at separate positions off the column. The smallest protease was found to “nick” the single chain intracellular tetanus toxin, producing the extracellular, tw...

Anti-Tetanus Serum

Lower Serum Levels of Antibodies against Tetanus Toxin in Patients with Hypothyroidism

Background: A fundamental duty of the immune system is to defend against infectious agents. Significant abnormalities were reported in immune parameters of hypothyroid and hyperthyroid patients. In this study, we aim to assess various quantities of antibodies against the tetanus toxin (anti-TT) in hypothyroid and hyperthyroid patients.  Materials and Methods: Anti-TT levels were measured in se...